I've never worked on a 543 but if its like most Bobcats just find where the fuel pick up line goes into the tank. There's also a return line so make sure your working with pick up line. It will run thru a rubber bushing, pry the bushing out along with the tubing. To check it, it should be longer than the tank is deep. If its never been replaced it would have had a screen on the end. Mine was magnetic and I was able to fish it out of the tank with a magnet attached to a piece of 1/4” rod.
You should use a new bushing when replacing the line. The bushing and line doesn't cost very much from the dealer. There's some debate on the forum as to whether or not you should re-install the screen so that's up to you.
Here's what it looks like on a 453.